6-磷酸葡萄糖脱氢酶(大肠杆菌)150单位 爱尔兰 型号:
JKY/E-PGDHEC库号:M230023 外观:悬浮液
在水中的溶解性:易溶。
pH值:6.0
气味:无
状态:液体
保质期:在冰箱5年或以上
成分:
名称 比例
6磷酸葡萄糖脱氢酶 1%
硫酸铵 45%
叠氮钠 0.02%
水 54%
EC 1.1.1.44. Recombinant from E. coli. In 3.2 M
ammonium sulphate. Specific activity: 21.4 U/mg (25oC, pH 7.6). Contaminants:
NADH oxidase = 0.00015 %, NADPH oxidase < 0.001 %. Stable at 4oC for > 2
years.
PROPERTIES
1. ELECTROPHORETIC PURITY
- Single band on
SDS-gel electrophoresis (MW ~ 52,546)
- Single major band on isoelectric
focusing (pI ~ 5.3)
2. SPECIFIC ACTIVITY AND LEVEL OF OTHER
ACTIVITIES
21.4 U/mg protein at pH 7.6 and 25°C.
One Unit of
6-phosphogluconate dehydrogenase (6-PGDH) is defined as the amount
of enzyme
required to produce one μmole of NADPH from NADP+
under the
following
assay conditions:
TEA buffer, pH 7.6 86 mM
ATP 7.5 mM
MgCl
2 8.6
mM
D-Gluconic acid 3.2 mM
NADP+ 0.9 mM
Gluconate kinase 6.0 U/mL
3.
OTHER ACTIVITIES (as a percentage of 6-PGDH activity)
Enzyme measured
Substrate Activity, %
6-PGDH D-gluconate 6-phosphate 100
NADH oxidase NADH
0.00015
NADPH oxidase NADPH 0.000000
4. PHYSICOCHEMICAL
PROPERTIES:
Recommended conditions of use are at pH 7.6 and up to 25°C.
5.
STORAGE AND USE CONDITIONS/RECOMMENDATIONS:
The enzyme is supplied as an
ammonium sulphate suspension and should be stored at
4°C. Swirl to mix the
enzyme suspension immediately prior to use