D-苹果酸检测试剂盒/D-Malic Acid Kit 100次爱尔兰

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产品名称：D-苹果酸检测试剂盒/D-Malic Acid Kit 100次爱尔兰
产品型号：JKY/K-DMAL
产品价格：2
所属类别：其他仪器仪表
发布时间：2017-05-05
产品产地：爱尔兰
已获点击：216

详细说明

D-苹果酸检测试剂盒/D-Malic Acid Kit 100次爱尔兰 型号:JKY/K-DMAL

库号：M245828

用于饮料和食品中对D-苹果酸（D-苹果酸）的特定检测。
成分
名称比例
甘氨酰甘氨酸缓冲液 1瓶（玻璃）
烟酰胺腺嘌呤二核苷酸 2瓶（玻璃）
D-苹果酸脱氢酶酶 1瓶（玻璃）
D-苹果酸标准品 1瓶（玻璃）

D-Malic Acid
For the specific assay of D-malic acid (D-malate) in beverages and food products.

Catalogue Number: K-DMAL
Content: 100 assays per kit

Appearance Five glass vials.
Specific Gravity not applicable
Solubility in Water Readily soluble.
pH Value 7.0
Odour none
Form powders and liquids
Stability stable in a freezer for five or more years
Ingredients Name CAS Proportion
Glycylglycine buffer 1 vial (glass)
Nicotinamide adenine dinucleotide 2 vials (glass)
D-Malate dehydrogenase enzyme 1 vial (glass)
D-Malic acid standard 1 vial (glass)
KITS:
Kits suitable for performing 50 determinations are available from
Megazyme. The kits contain the full assay method plus:
Bottle 1: Glycylglycine buffer (25 mL, 1 M, pH 8.0) plus
potassium chloride (0.5 M), magnesium chloride
(0.5 M) and sodium azide (0.02 % w/v) as a
preservative. Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+ (250 mg). Stable for > 5 years at -20°C.
Bottle 3: D-Malate dehydrogenase suspension
(2.2 mL, 220 U/mL). Stable for > 2 years at 4°C.
Bottle 4: D-Malic acid standard solution (5 mL, 0.20 mg/mL).
Stable for > 2 years at room temperature.
PREPARATION OF REAGENT SOLUTIONS/SUSPENSIONS:
1. Use the contents of bottle 1 as supplied.
Stable for > 2 years at 4°C.
2. Dissolve the contents of bottle 2 in 11 mL of distilled water.
Divide into appropriately sized aliquots and store in
polypropylene tubes at -20°C between use and on ice during
use. Once dissolved, the reagent is stable for > 2 years at
-20°C.
3. Use the contents of bottle 3 as supplied. Before opening for
the first time, shake the bottle to remove any enzyme that
may have settled on the rubber stopper. Subsequently, store
the bottle in an upright position. Swirl the bottle to mix
contents before use. Stable for > 2 years at 4°C.
4. Use the contents of bottle 4 as supplied.
Stable for > 2 years at room temperature.
3
NOTE: The D-malic acid standard solution is only assayed where
there is some doubt about the accuracy of the spectrophotometer
being used or where it is suspected that inhibition is being caused by
substances in the sample. The concentration of D-malic acid is
determined directly from the extinction coefficient of NADH
(see page 6).
4
EQUIPMENT (RECOMMENDED):
1. Volumetric flasks (50 mL and 100 mL).
2. Disposable plastic cuvettes (1 cm light path, 3.0 mL).
3. Micro-pipettors, e.g. Gilson Pipetman® (20 μL and 200 μL).
4. Positive displacement pipettor e.g. Eppendorf Multipette®
- with 5.0 mL Combitip® (to dispense 0.2 mL aliquots of NAD+
solution and of glycylglycine buffer).
- with 25 mL Combitip® (to dispense 2.0 mL aliquots of distilled
water).
5. Analytical balance.
6. Spectrophotometer set at 340 nm.
7. Vortex mixer (e.g. IKA®Yellowline Test Tube Shaker TTS2).
8. Stop clock.
9. Whatman No. 1 (9 cm) filter papers

东方化玻(北京)科技有限公司

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